Key features and details
- Rabbit polyclonal to SMC5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMC5 antibody
See all SMC5 primary antibodies
DescriptionRabbit polyclonal to SMC5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human RT4 cell lysate; Human prostate tissue; HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
RelevanceStructural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
Cellular localizationNucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
- hSMC 5 antibody
- hSMC5 antibody
- KIAA0594 antibody
Anti-SMC5 antibody (ab185373) at 1/250 dilution + Human RT4 cell lysate
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SMC5 using ab185373 at 1/200 dilution.
Immunofluorescence analysis of PFA/Triton X-100 permeabilized HeLa cells labeling SMC5 using ab185373 at 4 µg/ml.
ab185373 has been referenced in 1 publication.
- Wang D et al. Hydrogen gas inhibits lung cancer progression through targeting SMC3. Biomed Pharmacother 104:788-797 (2018). WB . PubMed: 29852353