Overview

  • Product name
  • Description
    Rabbit polyclonal to SMC6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human SMC6 aa 526-776 (internal sequence).
    Database link: Q96SB8

  • Positive control
    • NT2D1, IMR32 whole cell lysate; ES2 xenograft; MCF7 cells
  • General notes
    Keep as a concentrated solution.

    Previously labelled as SMC6L1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab155495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 126 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
  • Tissue specificity
    Widely expressed. Overexpressed in testis.
  • Sequence similarities
    Belongs to the SMC family. SMC6 subfamily.
  • Domain
    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.
  • Post-translational
    modifications
    Sumoylated by NSMCE2/MMS21.
    Ubiquitinated.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > telomere. Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22116 antibody
    • FLJ35534 antibody
    • hSMC6 antibody
    • SMC 6 antibody
    • SMC protein 6 antibody
    • SMC-6 antibody
    • SMC6 antibody
    • SMC6 protein antibody
    • SMC6 structural maintenance of chromosomes 6 like 1 antibody
    • SMC6_HUMAN antibody
    • Smc6l1 antibody
    • Structural maintenance of chromosomes 6 antibody
    • Structural maintenance of chromosomes 6 like 1 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    see all

Images

  • Anti-SMC6 antibody (ab155495) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 126 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling SMC6 with ab155495 at 1/200 dilution. Lower panel co-stained with Hoescht 33342.

  • Immunohistochemical analysis of paraffin-embedded ES2 xenograft tissue labeling SMC6 with ab155495 at 1/500 dilution.

References

ab155495 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155495.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up