Key features and details
- Rabbit polyclonal to SMC6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMC6 antibody
See all SMC6 primary antibodies
DescriptionRabbit polyclonal to SMC6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human SMC6 aa 526-776 (internal sequence).
Database link: Q96SB8
- NT2D1, IMR32 whole cell lysate; ES2 xenograft; MCF7 cells
General notesKeep as a concentrated solution.
Previously labelled as SMC6L1.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 126 kDa.|
|IHC-P||1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionCore component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
Tissue specificityWidely expressed. Overexpressed in testis.
Sequence similaritiesBelongs to the SMC family. SMC6 subfamily.
DomainThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.
modificationsSumoylated by NSMCE2/MMS21.
Cellular localizationNucleus. Chromosome. Chromosome > telomere. Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
- Information by UniProt
- FLJ22116 antibody
- FLJ35534 antibody
- hSMC6 antibody
Anti-SMC6 antibody (ab155495) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 126 kDa
7.5% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling SMC6 with ab155495 at 1/200 dilution. Lower panel co-stained with Hoescht 33342.
Immunohistochemical analysis of paraffin-embedded ES2 xenograft tissue labeling SMC6 with ab155495 at 1/500 dilution.
ab155495 has not yet been referenced specifically in any publications.