Anti-SMCHD1 antibody (ab122555)
Key features and details
- Rabbit polyclonal to SMCHD1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMCHD1 antibody
See all SMCHD1 primary antibodies -
Description
Rabbit polyclonal to SMCHD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: DNGRGMTSKQ LNNWAVYRLS KFTRQGDFES DHSGYVRPVP VPRSLNSDIS YFGVGGKQAV FFVGQSARMI SKPADSQDVH ELVLSKED, corresponding to N terminal amino acids 184-271 of Human SMCHD1.
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Positive control
- WB: U 2 OS and MCF7 cell lysates; IHC-P: Human duodenum tissue; ICC/IF: Human cell like U-2 OS.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 23347 Human
- Omim: 614982 Human
- SwissProt: A6NHR9 Human
- Unigene: 8118 Human
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Alternative names
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows localization to nucleoplasm. Recommended concentration of ab122555 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122555, at 1/50 dilution, staining SMCHD1 in Paraffin Embedded human duodenum shows nucelar positivity in glandular cells by Immunohistochemistry.
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All lanes : Anti-SMCHD1 antibody (ab122555) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysateLoading Control: Anti-HDAC1
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab122555 has been referenced in 5 publications.
- Cheng J et al. Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription. J Virol 94:N/A (2020). PubMed: 32581092
- Ruebel ML et al. SMCHD1 terminates the first embryonic genome activation event in mouse two-cell embryos and contributes to a transcriptionally repressive state. Am J Physiol Cell Physiol 317:C655-C664 (2019). PubMed: 31365290
- Midic U et al. Novel key roles for structural maintenance of chromosome flexible domain containing 1 (Smchd1) during preimplantation mouse development. Mol Reprod Dev 85:635-648 (2018). PubMed: 29900695
- Shaw ND et al. SMCHD1 mutations associated with a rare muscular dystrophy can also cause isolated arhinia and Bosma arhinia microphthalmia syndrome. Nat Genet 49:238-248 (2017). PubMed: 28067909
- Sunwoo H et al. The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells. Proc Natl Acad Sci U S A 112:E4216-25 (2015). Mouse . PubMed: 26195790