Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
- Information by UniProt
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
All lanes : Anti-SMCHD1 antibody (ab122555) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG
Lane 3 : Human Plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122555 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122555, at 1/50 dilution, staining SMCHD1 in Paraffin Embedded Human testis tissue by Immunohistochemistry.
This product has been referenced in:
- Ruebel ML et al. SMCHD1 terminates the first embryonic genome activation event in mouse two-cell embryos and contributes to a transcriptionally repressive state. Am J Physiol Cell Physiol 317:C655-C664 (2019). Read more (PubMed: 31365290) »
- Midic U et al. Novel key roles for structural maintenance of chromosome flexible domain containing 1 (Smchd1) during preimplantation mouse development. Mol Reprod Dev 85:635-648 (2018). Read more (PubMed: 29900695) »