Key features and details
- Rabbit polyclonal to SMCHD1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMCHD1 antibody
See all SMCHD1 primary antibodies
DescriptionRabbit polyclonal to SMCHD1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human SMCHD1 aa 250-300. The exact sequence is proprietary. NP_056110.2
Database link: A6NHR9
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma and non-small cell lung cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245598 was affinity purified using an epitope specific to SMCHD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 226 kDa.|
- Information by UniProt
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
Formalin-fixed, paraffin-embedded human testicular seminoma tissue (left panel) and non-small cell lung cancer tissue (right panel) stained for SMCHD1 using ab245598 at 1/200 dilution in immunohistochemical analysis. Detection: Commercial peroxidase substrate.
All lanes : Anti-SMCHD1 antibody (ab245598) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 226 kDa
Exposure time: 3 minutes
SMCHD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245598 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245598 at 0.4 µg/ml.
Lane 1: ab245598 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245598 has not yet been referenced specifically in any publications.