Overview

  • Product name

  • Description

    Rabbit polyclonal to SMCHD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SMCHD1 aa 250-300. The exact sequence is proprietary. NP_056110.2
    Database link: A6NHR9

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma and non-small cell lung cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245598 was affinity purified using an epitope specific to SMCHD1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 226 kDa.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • BAMS antibody
    • FSHD2 antibody
    • KIAA0650 antibody
    • SMC hinge domain containing 1 antibody
    • SMC hinge domain containing protein 1 antibody
    • Smchd1 antibody
    • SMHD1_HUMAN antibody
    • Structural maintenance of chromosomes flexible hinge domain containing 1 antibody
    • Structural maintenance of chromosomes flexible hinge domain-containing protein 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human testicular seminoma tissue (left panel) and non-small cell lung cancer tissue (right panel) stained for SMCHD1 using ab245598 at 1/200 dilution in immunohistochemical analysis. Detection: Commercial peroxidase substrate.

  • All lanes : Anti-SMCHD1 antibody (ab245598) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 226 kDa


    Exposure time: 3 minutes
  • SMCHD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245598 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245598 at 0.4 µg/ml.

    Lane 1: ab245598 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab245598 has not yet been referenced specifically in any publications.

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