Overview

  • Product name

    Anti-SMCHD1 antibody [EPR12340-29]
    See all SMCHD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12340-29] to SMCHD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human SMCHD1 aa 1800-2000. The exact sequence is proprietary.
    Database link: A6NHR9

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12340-29
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

WB 1/1000 - 1/10000. Predicted molecular weight: 226 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Information by UniProt
    • Database links

    • Alternative names

      • BAMS antibody
      • FSHD2 antibody
      • KIAA0650 antibody
      • SMC hinge domain containing 1 antibody
      • SMC hinge domain containing protein 1 antibody
      • Smchd1 antibody
      • SMHD1_HUMAN antibody
      • Structural maintenance of chromosomes flexible hinge domain containing 1 antibody
      • Structural maintenance of chromosomes flexible hinge domain-containing protein 1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue, labeling SMCHD1 using ab179456 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue, labeling SMCHD1 using ab179456 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-SMCHD1 antibody [EPR12340-29] (ab179456) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 226 kDa

    References

    This product has been referenced in:

    • Hiramuki Y & Tapscott SJ Identification of SMCHD1 domains for nuclear localization, homo-dimerization, and protein cleavage. Skelet Muscle 8:24 (2018). Read more (PubMed: 30071896) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179456.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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