Overview

  • Product name

  • Description

    Rabbit polyclonal to SMCR8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 279-396 of Human SMCR8.

  • Positive control

    • Human rectum tissue; RT 4, U 251 MG, and Human Tonsil lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

Images

  • ab126821 at 1/150 dilution staining SMCR8 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli using ab121682.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References

This product has been referenced in:

  • Yang M  et al. A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. Sci Adv 2:e1601167 (2016). Mouse . Read more (PubMed: 27617292) »
See 1 Publication for this product

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