Overview

  • Product name

  • Description

    Rabbit polyclonal to SMCR8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human SMCR8 aa 600-650 (internal sequence). The exact sequence is proprietary. NP_658988.2
    Database link: Q8TEV9

  • Positive control

    • NIH 3T3 whole cell lysate (ab7179) can be used as a positive control in WB. Whole cell lysate from HeLa, 293T, Jurkat, and mouse TCMK-1 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab202283 was affinity purified using an epitope specific to SMCR8 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.

Target

Images

  • All lanes : Anti-SMCR8 antibody (ab202283) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells
    Lane 2 : Whole cell lysate from 293T cells
    Lane 3 : Whole cell lysate from Jurkat cells
    Lane 4 : Whole cell lysate from mouse TCMK-1 cells
    Lane 5 : Whole cell lysate from mouse NIH 3T3 cells

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 105 kDa


    Exposure time: 3 minutes
  • Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of SMCR8.
    Lane 2: Detection of Human SMCR8 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202283 at 6 µg per reaction for IP.
    Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab202283 at 0.4 µg/ml.

     

    Detection: Chemiluminescence with an exposure time of 3
    minutes.

References

This product has been referenced in:

  • Zhang Y  et al. The C9orf72-interacting protein Smcr8 is a negative regulator of autoimmunity and lysosomal exocytosis. Genes Dev 32:929-943 (2018). Read more (PubMed: 29950492) »
  • Jung J  et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. Elife 6:N/A (2017). WB, ICC/IF ; Human . Read more (PubMed: 28195531) »
See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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