• Product name

  • Description

    Rabbit polyclonal to SMEK2
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.



Our Abpromise guarantee covers the use of ab70622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Relevance

    SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
  • Cellular localization

    Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
  • Database links

  • Alternative names

    • FLFL2 antibody
    • FLJ31474 antibody
    • KIAA1387 antibody
    • PP4R3 beta antibody
    • PP4R3B antibody
    • PPP4R3 Beta antibody
    • PPP4R3B antibody
    • Protein Phosphatase 4, Regulatory Subunit 3 Beta, antibody
    • PSY2 antibody
    • Serine/threonine-protein phosphatase 4 regulatory subunit 3B antibody
    • SMEK 2 antibody
    • SMEK homolog 2 antibody
    • SMEK homolog 2 suppressor of mek1 (Dictyostelium) antibody
    • SMEK homolog 2 suppressor of mek1 antibody
    • smk1 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMEK2 with ab70622 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.


This product has been referenced in:

  • Ho MY  et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). Read more (PubMed: 27391343) »
See 1 Publication for this product

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