• Product name

  • Description

    Rabbit polyclonal to Smg1
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan, Platypus
  • Immunogen

    Synthetic peptide within Human Smg1 aa 1250-1300. The exact sequence is proprietary.
    Database link: NP_055907.3

  • Positive control

    • HeLa cell lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab80600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 410 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
  • Tissue specificity

    Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 HEAT repeat.
    Contains 1 PI3K/PI4K domain.
  • Post-translational

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 61E3.4 antibody
    • ATX antibody
    • hSMG-1 antibody
    • hSMG1 antibody
    • KIAA0421 antibody
    • Lambda interacting protein antibody
    • Lambda-interacting protein antibody
    • Lambda/iota protein kinase C interacting protein antibody
    • Lambda/iota protein kinase C-interacting protein antibody
    • LIP antibody
    • Phosphatidylinositol 3 kinase related kinase antibody
    • Phosphatidylinositol 3 kinase related protein kinase antibody
    • PI 3 kinase related kinase SMG 1 antibody
    • Serine/threonine protein kinase SMG1 antibody
    • Serine/threonine-protein kinase SMG1 antibody
    • SMG-1 antibody
    • smg1 antibody
    • SMG1_HUMAN antibody
    see all


  • All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml

    Lane 1 : Whole HeLa cell lysate at 50 µg
    Lane 2 : Whole HeLa cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 410 kDa
    Observed band size: 410 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    Chemiluminescence with exposure times of 30 seconds
  • Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins


This product has been referenced in:

  • Henderson-Smith A  et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . Read more (PubMed: 24204929) »
See 1 Publication for this product

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