Key features and details
- Rabbit polyclonal to Smg1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Smg1 antibody
See all Smg1 primary antibodies
DescriptionRabbit polyclonal to Smg1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan, Platypus
Synthetic peptide within Human Smg1 aa 1250-1300. The exact sequence is proprietary.
Database link: NP_055907.3
- HeLa cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 410 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionSerine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
Tissue specificityWidely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 1 HEAT repeat.
Contains 1 PI3K/PI4K domain.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 61E3.4 antibody
- ATX antibody
- hSMG-1 antibody
All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg
Lane 2 : Whole HeLa cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 410 kDa
Observed band size: 410 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Chemiluminescence with exposure times of 30 seconds
Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins
ab80600 has been referenced in 1 publication.
- Henderson-Smith A et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . PubMed: 24204929