Recombinant Anti-Smg1 antibody [EPR4591(2)] (ab151730)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4591(2)] to Smg1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Smg1 antibody [EPR4591(2)]
See all Smg1 primary antibodies -
Description
Rabbit monoclonal [EPR4591(2)] to Smg1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Smg1 aa 3600-3700 (C terminal). The exact sequence is proprietary.
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Positive control
- Human fetal brain, Saos2, HT1080, Human skeletal muscle, HeLa and 293T lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4591(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 410 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 410 kDa. |
Target
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Function
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. -
Tissue specificity
Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 1 HEAT repeat.
Contains 1 PI3K/PI4K domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23049 Human
- Entrez Gene: 233789 Mouse
- Entrez Gene: 691397 Rat
- Omim: 607032 Human
- SwissProt: Q96Q15 Human
- SwissProt: Q8BKX6 Mouse
- Unigene: 460179 Human
- Unigene: 309053 Mouse
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Alternative names
- 61E3.4 antibody
- ATX antibody
- hSMG-1 antibody
see all
Images
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All lanes : Anti-Smg1 antibody [EPR4591(2)] (ab151730) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Saos2 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : Human skeletal muscle lysate
Lane 5 : HeLa cell lysate
Lane 6 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 410 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151730 has been referenced in 1 publication.
- Chu V et al. Selective destabilization of polypeptides synthesized from NMD-targeted transcripts. Mol Biol Cell 32:ar38 (2021). PubMed: 34586879