Product nameAnti-SMG5 antibody [EPR3503(2)]
See all SMG5 primary antibodies
DescriptionRabbit monoclonal [EPR3503(2)] to SMG5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human SMG5 aa 950-1050. The exact sequence is proprietary.
- HeLa, 293T, and K562 cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; and 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 114 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
RelevanceSMG5 plays a role in nonsense mediated mRNA decay. It promotes dephosphorylation of RENT1, and is necessary for TERT activity. It is part of a complex that contains SMG1, SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1/UPF1.
Cellular localizationCytoplasmic and Nuclear
- EST1 like protein B antibody
- EST1B antibody
- LPTS interacting protein antibody
All lanes : Anti-SMG5 antibody [EPR3503(2)] (ab129107) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 114 kDa
ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
ab129107, at 1/100 dilution, staining SMG5 in Paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
ab129107, at 1/100 dilution, staining SMG5 in 293T cells by Immunofluorescence.
This product has been referenced in:
- Cheruiyot A et al. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS One 13:e0204978 (2018). Read more (PubMed: 30289931) »
- Hu J et al. MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1. PLoS One 8:e68291 (2013). WB ; Human . Read more (PubMed: 23826386) »