Product nameAnti-SMG6 antibody
See all SMG6 primary antibodies
DescriptionRabbit polyclonal to SMG6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
Synthetic peptide corresponding to Human SMG6 aa 1400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both Jurkat and HepG2 nuclear lysates.
This product was previously labelled as EST1A
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24554440|
RelevanceTelomerase-binding protein EST1A is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It has been shown to promote the ability of TERT to elongate telomeres and may have some endonuclease function.
- C17orf31 antibody
- EST1-like protein A antibody
- Ever shorter telomeres 1A antibody
All lanes : Anti-SMG6 antibody (ab87539) at 1 µg/ml
Lane 1 :
Jurkat nuclear extract lysate (ab14844)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted Mw for Human Telomerase-binding protein SMG6 is 160 kDa. We are unsure why we are seeing the banding pattern at kDa however Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab87539 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab87539, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100% methanol fixed (5 min) MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data
This product has been referenced in:
- Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). Read more (PubMed: 29334995) »
- Cheruiyot A et al. Compound C inhibits nonsense-mediated RNA decay independently of AMPK. PLoS One 13:e0204978 (2018). Read more (PubMed: 30289931) »