Western blot abreview for Anti-SMG6 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Polyclonal Goat Anti- Rabbit Immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
Knockout ESCs (Genotyped as Smg6 -/-) Lane 5-8
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: around 190 and 130 kDa
Positive control
Wild type ESCs (Genotyped as Smg6 F/F) Lane 1-4

Additional data

Additional Notes
membrane was cutted around 72KD. Possible Smg6 products less than 72KD could not be shown in this blot.

Dr. Tangliang Li

Verified customer

Submitted May 11 2015

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