Key features and details
- Rabbit polyclonal to SMG9
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMG9 antibody
DescriptionRabbit polyclonal to SMG9
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human SMG9.
Database link: NP_061981.2
- HeLa cell lysate
Previously labelled as SMG9.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
Not suitable for crude preparations (e.g. whole cell lysate). Can be used for WB of enriched (e.g. immunoprecipitated) sources of SMG9.
FunctionInvolved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8.
Sequence similaritiesBelongs to the SMG9 family.
modificationsPhosphorylated by SMG1.
- Information by UniProt
- C19orf61 antibody
- Chromosome 19 open reading frame 61 antibody
- DKFZp564H1322 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMG9 with ab85689 at 1/5000 (0.2µg/ml). Detection: DAB.
ab85659 has been referenced in 1 publication.
- Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). PubMed: 29334995