Anti-SMNDC1 antibody (ab227952)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SMNDC1 antibody
See all SMNDC1 primary antibodies -
Description
Rabbit polyclonal to SMNDC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human SMNDC1 (internal sequence). The exact sequence is proprietary.
Database link: O75940 -
Positive control
- IHC-P: Human lung cancer tissue; C2C12 and SNU1 xenograft tissues. WB: IMR32 whole cell lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 27 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Necessary for spliceosome assembly. Overexpression causes apoptosis. -
Tissue specificity
Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas. -
Sequence similarities
Belongs to the SMN family.
Contains 1 Tudor domain. -
Cellular localization
Nucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies. - Information by UniProt
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Database links
- Entrez Gene: 520500 Cow
- Entrez Gene: 10285 Human
- Entrez Gene: 76479 Mouse
- Entrez Gene: 287768 Rat
- Omim: 603519 Human
- SwissProt: Q3T045 Cow
- SwissProt: O75940 Human
- SwissProt: Q8BGT7 Mouse
see all -
Alternative names
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
see all
Images
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Western blot - Anti-SMNDC1 antibody (ab227952)Anti-SMNDC1 antibody (ab227952) at 1/1000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SMNDC1 antibody (ab227952)Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for SMNDC1 (green) using ab227952 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded human lung cancer tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded C2C12 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded SNU1 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
References
ab227952 has not yet been referenced specifically in any publications.
