Key features and details
- Rabbit polyclonal to SMNDC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SMNDC1 antibody
See all SMNDC1 primary antibodies
DescriptionRabbit polyclonal to SMNDC1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
Recombinant fragment within Human SMNDC1 (internal sequence). The exact sequence is proprietary.
Database link: O75940
- IHC-P: Human lung cancer tissue; C2C12 and SNU1 xenograft tissues. WB: IMR32 whole cell lysate. ICC/IF: MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionNecessary for spliceosome assembly. Overexpression causes apoptosis.
Tissue specificityDetected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.
Sequence similaritiesBelongs to the SMN family.
Contains 1 Tudor domain.
Cellular localizationNucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.
- Information by UniProt
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
Anti-SMNDC1 antibody (ab227952) at 1/1000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa
12% SDS-PAGE gel.
Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for SMNDC1 (green) using ab227952 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
Paraffin-embedded human lung cancer tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded C2C12 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded SNU1 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227952 has not yet been referenced specifically in any publications.