Anti-SMNDC1 antibody (ab227952)
Key features and details
- Rabbit polyclonal to SMNDC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMNDC1 antibody
See all SMNDC1 primary antibodies -
Description
Rabbit polyclonal to SMNDC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human SMNDC1 (internal sequence). The exact sequence is proprietary.
Database link: O75940 -
Positive control
- IHC-P: Human lung cancer tissue; C2C12 and SNU1 xenograft tissues. WB: IMR32 whole cell lysate. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 27 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Necessary for spliceosome assembly. Overexpression causes apoptosis. -
Tissue specificity
Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas. -
Sequence similarities
Belongs to the SMN family.
Contains 1 Tudor domain. -
Cellular localization
Nucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies. - Information by UniProt
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Database links
- Entrez Gene: 520500 Cow
- Entrez Gene: 10285 Human
- Entrez Gene: 76479 Mouse
- Entrez Gene: 287768 Rat
- Omim: 603519 Human
- SwissProt: Q3T045 Cow
- SwissProt: O75940 Human
- SwissProt: Q8BGT7 Mouse
see all -
Alternative names
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
see all
Images
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Western blot - Anti-SMNDC1 antibody (ab227952)Anti-SMNDC1 antibody (ab227952) at 1/1000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SMNDC1 antibody (ab227952)Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for SMNDC1 (green) using ab227952 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded human lung cancer tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded C2C12 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMNDC1 antibody (ab227952)Paraffin-embedded SNU1 xenograft tissue stained for SMNDC1 using ab227952 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227952 has not yet been referenced specifically in any publications.
