Key features and details
- Rabbit polyclonal to SMNDC1
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMNDC1 antibody
See all SMNDC1 primary antibodies
DescriptionRabbit polyclonal to SMNDC1
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Recombinant full length protein corresponding to Human SMNDC1 aa 1-238.
MSEDLAKQLASYKAQLQQVEAALSGNGENEDLLKLKKDLQEVIELTKDLL STQPSETLASSDSFASTQPTHSWKVGDKCMAVWSEDGQCYEAEIEEIDEE NGTAAITFAGYGNAEVTPLLNLKPVEEGRKAKEDSGNKPMSKKEMIAQQR EYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFA SPESVTGKVGVGTCGIADKPMTQYQDTSKYNVRHLMPQ
Database link: O75940
- IHC-P: Human heart tissue. IP: HeLa whole cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
FunctionNecessary for spliceosome assembly. Overexpression causes apoptosis.
Tissue specificityDetected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.
Sequence similaritiesBelongs to the SMN family.
Contains 1 Tudor domain.
Cellular localizationNucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.
- Information by UniProt
- 30 kDa splicing factor SMNrp antibody
- MGC106917 antibody
- MGC112663 antibody
Paraffin-embedded human heart tissue stained for SMNDC1 using ab238846 at 1/100 dilution in immunohistochemical analysis.
SMNDC1 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab238846 at 1/200 dilution.
Lane 1: Rabbit control IgG instead of ab238846 in HeLa whole cell lysate.
Lane 2: ab238846 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 μg, input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238846 has not yet been referenced specifically in any publications.