Question (9466) | Anti-SMN/Gemin 1 antibody [2B1] (ab5831)

Go to datasheet (ab5831)

Question

what is the starting dilution for this antibody when used for Western? thank you

Answer

Thank you for your enquiry. The datasheet does not recommend a specific dilution as it may vary between preparations/tissues and also depending on the amount of gemin 1 in your sample and the detection system you use. I would recommend a preliminary experiment to determine an optimal concentration: try a few dilutions of 1:500, 1:1000, 1:2000. Then optimise the dilution depending on those results. Good luck with your research,

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