Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 1 antibody [2B1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C

Other product details

Dilution
1/300
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2 % Fish scale gelatin in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Gemin 1 is clearly visualized (green channel) in the cytoplasm and also as small dots in nuclear (Cajal bodies?) of Hela cells by immunostaining using Gemin 1 antibody [2B1] (ab5831)

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Submitted Apr 26 2011

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