Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 1 antibody [2B1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (ES cells)
Specification
ES cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin in PBS as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C

Other product details

Dilution
1/300
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2 % Fish scale gelatin in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Gemin 1 is clearly visualized (green channel) in the cytoplasm of mouse ES cells by immunostaining using Anti-Gemin 1 antibody [2B1] (a b5831).

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Submitted Jul 24 2012

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