Western blot abreview for Anti-Gemin 1 antibody [2B1]

Good
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (st II-III oocytes)
Gel Running Conditions
Reduced Denaturing (4-20% precast)
Loading amount
20 cells
Specification
st II-III oocytes
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/25000
Name

Detection

Detection method
Supersignal Femto ECL
Exposure
30 second(s)
Bands
Specific: 31 kDa

Additional data

Additional Notes
Specific antibody, but high concentration of antibody required for detection

Ms. Samantha Jeschonek

Verified customer

Submitted Feb 03 2017

Sign up