RecombinantRabMAb

Recombinant Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)

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Western blot - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
  • Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4430] to SMN/Gemin 1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free
    See all SMN/Gemin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4430] to SMN/Gemin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SMN/Gemin 1 aa 150-250. The exact sequence is proprietary.

  • General notes

    Ab247668 is the carrier-free version of ab108424. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247668 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.

    • Tissue specificity

      Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).

    • Involvement in disease

      Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 1 (SMA1) [MIM:253300]. Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. Autosomal recessive forms are classified according to the age of onset, the maximum muscular activity achieved, and survivorship. The severity of the disease is mainly determined by the copy number of SMN2, a copy gene which predominantly produces exon 7-skipped transcripts and only low amount of full-length transcripts that encode for a protein identical to SMN1. Only about 4% of SMA patients bear one SMN1 copy with an intragenic mutation. SMA1 is a severe form, with onset before 6 months of age. SMA1 patients never achieve the ability to sit.
      Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 2 (SMA2) [MIM:253550]. SMA2 is an autosomal recessive spinal muscular atrophy of intermediate severity, with onset between 6 and 18 months. Patients do not reach the motor milestone of standing, and survive into adulthood.
      Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 3 (SMA3) [MIM:253400]. SMA3 is an autosomal recessive spinal muscular atrophy with onset after 18 months. SMA3 patients develop ability to stand and walk and survive into adulthood.
      Defects in SMN1 are the cause of spinal muscular atrophy autosomal recessive type 4 (SMA4) [MIM:271150]. SMA4 is an autosomal recessive spinal muscular atrophy characterized by symmetric proximal muscle weakness with onset in adulthood and slow disease progression. SMA4 patients can stand and walk.

    • Sequence similarities

      Belongs to the SMN family.
      Contains 1 Tudor domain.

    • Cellular localization

      Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BCD541 antibody
      • Component of gems 1 antibody
      • Gemin 1 antibody
      • Gemin-1 antibody
      • OTTHUMP00000125198 antibody
      • OTTHUMP00000223567 antibody
      • OTTHUMP00000223568 antibody
      • OTTHUMP00000224066 antibody
      • OTTHUMP00000226924 antibody
      • SMA 1 antibody
      • SMA 2 antibody
      • SMA 3 antibody
      • SMA 4 antibody
      • SMA antibody
      • SMA@ antibody
      • SMA1 antibody
      • SMA2 antibody
      • SMA3 antibody
      • SMA4 antibody
      • SMN antibody
      • SMN_HUMAN antibody
      • SMN1 antibody
      • SMN2 antibody
      • SMNT antibody
      • Survival motor neuron protein antibody
      • Survival of motor neuron 1, telomeric antibody
      • T-BCD541 antibody
      see all

    Images

    • Western blot - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
      Western blot - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
      All lanes : Anti-SMN/Gemin 1 antibody [EPR4430] (ab108424) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa



      This data was developed using ab108424, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
      This data was developed using ab108424, the same antibody clone in a different buffer formulation.ab108424 staining Gemin 1 in paraffin-embedded Human kidney tissue by Immunohistochemistry at dilution of 1:500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)
      Anti-SMN/Gemin 1 antibody [EPR4430] - BSA and Azide free (ab247668)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247668? Please let us know so that we can cite the reference in this datasheet.

    ab247668 has not yet been referenced specifically in any publications.

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