Overview

  • Product name
  • Description
    Rabbit polyclonal to SMOC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 140-363 of Human SMOC1 (Uniprot ID Q9H4F8).

  • Positive control
    • IMR32 whole cell lysate; Human colon cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab155776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays essential roles in both eye and limb development. Probale regulator of osteoblast differentiation.
  • Tissue specificity
    Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
  • Involvement in disease
    Defects in SMOC1 are the cause of ophthalmoacromelic syndrome (OAS) [MIM:206920]. A rare disorder presenting with anophthalmia or microphthalmia and limb anomalies. Limb malformations include fused 4th and 5th metacarpals and short 5th finger in hands, and olygodactyly in foot (four toes). Most patients have bilateral anophthalmia/ microphthalmia, but unilateral abnormality is also noted. Other malformations are rare, but venous or vertebral anomaly was recognized each in single cases.
  • Sequence similarities
    Contains 2 EF-hand domains.
    Contains 1 Kazal-like domain.
    Contains 2 thyroglobulin type-1 domains.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Secreted modular calcium-binding protein 1 antibody
    • SMOC-1 antibody
    • SMOC1 antibody
    • SMOC1_HUMAN antibody
    • SPARC-related modular calcium-binding protein 1 antibody
    see all

Images

  • Anti-SMOC1 antibody (ab155776) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling SMOC1 with ab155776 at 1/250 dilution.

References

ab155776 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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