Overview

  • Product name
    Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description
    Mouse monoclonal [1G12] to smooth muscle Myosin heavy chain 11
  • Host species
    Mouse
  • Specificity
    This antibody reacts with human, mouse, and rat smooth muscle myosin heavy chain 11 (SM2). Does not cross-react with smooth muscle Myosin heavy chain 1.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11. The immunogen sequence exists in isoforms 3 and 4 but not isoforms 1 and 2 of the UniProt record P35749.
    Sequence:

    RGPPPQETSQ


    Database link: P35749-3

Properties

Applications

Our Abpromise guarantee covers the use of ab683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. PubMed: 21103378
IHC-Fr 1/400.
ICC/IF 1/250.
IHC-P 1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin.

Target

  • Function
    Muscle contraction.
  • Tissue specificity
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor® 594 conjugated goat anti-mouse IgG1 was used as the secondary.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in rat cells from cells from mesenteric artery by ICC/IF. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% serum for 30 minutes at 20°C. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4°C. Alexa Fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary. Nuclei stained with Hoechst 33342.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in human cerebral artery tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton-X and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with the primary antibody (1/500) for 16 hours at 4°C. A biotin-conjugated rabbit anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain in human freshly isolated arterial myocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 30 minutes at 20°C. Samples were incubated with the primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated chicken anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Li Z  et al. Upregulation of Oxytocin Receptor in the Hyperplastic Prostate. Front Endocrinol (Lausanne) 9:403 (2018). Read more (PubMed: 30123183) »
  • Zhou B  et al. Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury. Arterioscler Thromb Vasc Biol 37:675-684 (2017). Read more (PubMed: 28153879) »
See all 29 Publications for this product

Customer reviews and Q&As

21-30 of 36 Abreviews or Q&A

Answer

Thank you for your reply.


For ab683, I'd be happy to offer you the following:

Either a refund or credit for ab683 plus another antibody free of charge, or alternatively 2 antibodies of your choice free of charge. Please let me know which option you would prefer.

Currently, we only carry antibodies to smooth muscle myosin heavy chain 1 and 2. We have 7 of those: ab77967, ab82255, ab97539, ab124679, ab81031, ab106919, and ab17811. Please let me know as well if any of these antibodies would be of interest to you.

As for ab8969, please send me an email with more experimental details (such as antibody dilutions, results obtained, detection system used, sample type used (cultured cell or tissue sections), etc.), and I'd be happy to look into this.

I look forward to hear back from you and assist you further.

Read More

Answer

Thank you for your email.

Yes, that is no problem at all.

I am sorry that the product ab683 had this mistake on its datasheet - which has been corrected by now - and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with 2x ab7481.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting us.
I'd be happy to send you an anti-smooth muscle Myosin heavy chain 2 antibody free of charge.
We currently only have 7 "Anti-Myosin, smooth muscle heavy chain 1 and 2" antibodies in our catalog:
ab82255, ab77967, ab97539, ab124679, ab81031, ab106919, and ab17811
If any of these would be suitable for you, please let me know which application and species you are working with. I will then arrange for the shipment.
I look forward to hear back from you and assist you further.

Read More

Answer

Thank you for contacting us.

Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry for the mistake on the datasheet forthis antibody. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for your call today.
I apologize for the inconvenience with ab683, and as we discussed I would be happy to send a replacement or issue a credit or refund. Also, please keep me updated about any further information about ab21108.
I look forward to hearing from you. Have a great day.

Read More

Answer

The anti-smooth muscle Myosin heavy chain 2 antibody [1G12], ab682 recognizes the human SM1 isoform which is a 204kDa protein. If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for contacting Abcam about ab683. The gene for the anti-smooth muscle Myosin heavy chain 2 antibody [1G12] (ab683) is MYH11. The link below will take you to the SwissProt ID#P35748, which contains the information for the protein/gene that ab683 recognizes: http://www.uniprot.org/uniprot/P35748 If there is anything else I can help with, please let me know.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human Cerebral Artery)
Specification
Human Cerebral Artery
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 19 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Rhesus Macaque Cerebral Artery)
Specification
Rhesus Macaque Cerebral Artery
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - Triton-X 0.3%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2011

21-30 of 36 Abreviews or Q&A

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