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WB with human vascular smooth muscle cells
sees bands at 125 and 75, or at 60 kDa - but nothing at 200 kDa
Ab: 1/500, 1/1000
block: 5% casein or BSA
ladder: 250 - 10 kDa, sees 250 kDa band from ladder
loaded 20 ug protein
2nd: works fine
Asked on Oct 08 2012
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Answered on Oct 08 2012