Immunocytochemistry/ Immunofluorescence abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (smooth muscle cells from mesenteric artery)
Specification
smooth muscle cells from mesenteric artery
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Other product details

Dilution
1/300
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PSS with 2%BSA and 0.3% Triton X-100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 Chicken anti-mouse IgG (Invitroge
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
Fixation 4% paraformaldehyde in physiological saline solution (PSS) 4 min at 4°C,
Nuclei stained with Hoechst 33342

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Submitted Nov 16 2009

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