Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Cerebral Artery)
Specification
Cerebral Artery
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - Triton-X 0.3%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-mouse biotinilated
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300

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Submitted Mar 09 2011

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