Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100 for 15 mins
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS + 0.05% Tween-20
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 647 Goat anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

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Submitted Oct 20 2016

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