Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description

    Rabbit polyclonal to smooth muscle Myosin heavy chain 11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab153753)

  • Positive control

    • This antibody gave a positive signal in Western Blot within Mouse Bladder tissue lysate. This antibody also gave a positive signal in Immunohistochemistry within Human normal Heart Muscle formalin fixed paraffin embedded tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab125884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Muscle contraction.
  • Tissue specificity

    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease

    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • ab125884 staining smooth muscle Myosin heavy chain 11 in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of smooth muscle Myosin heavy chain 11 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125884, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-smooth muscle Myosin heavy chain 11 antibody (ab125884) at 1 µg/ml + Bladder (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 227 kDa
    Observed band size: 227 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Liao Q  et al. Irisin exerts a therapeutic effect against myocardial infarction via promoting angiogenesis. Acta Pharmacol Sin N/A:N/A (2019). Read more (PubMed: 31061533) »
  • MacFarlane EG  et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest 129:659-675 (2019). Read more (PubMed: 30614814) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (primary embryonic epicardial)
Specification
primary embryonic epicardial
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 29 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: antigen retrieval
Sample
Mouse Tissue sections (Heart - day 0 and 7 post-MI)
Specification
Heart - day 0 and 7 post-MI
Permeabilization
No
Fixative
10% zinc formalin

Dr. Merry Lindsey

Verified customer

Submitted Sep 06 2013

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Treatment
Day 0 and day 7 post MI LV homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Merry Lindsey

Verified customer

Submitted Sep 06 2013

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