Overview

  • Product name
    Anti-smooth muscle Myosin heavy chain 11 antibody
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description
    Rabbit polyclonal to smooth muscle Myosin heavy chain 11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human, Pig
    Predicted to work with: Vertebrata
  • Immunogen

    Bovine tracheal smooth muscle myosin.

Properties

Applications

Our Abpromise guarantee covers the use of ab53219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 227 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19729479
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Muscle contraction.
  • Tissue specificity
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • Lanes 3 and 4: Pig Tissue lysate - Heart

    See Abreview

  • Immuhistochemical analysis of paraffin-embedded sections of uterus labelling smoth muscle myosin chain 11 with ab53219 at 1/500 dilution.

    See Abreview

  • ab53219 staining smooth muscle Myosin heavy chain 11 in Pig aortic smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. Ab6717 (1/400) was used as the secondary antibody.

    See Abreview

  • ab53219 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

    See Abreview

References

This product has been referenced in:
  • Sarker MAK  et al. Class II PI3Ks a and ß Are Required for Rho-Dependent Uterine Smooth Muscle Contraction and Parturition in Mice. Endocrinology 160:235-248 (2019). Read more (PubMed: 30476019) »
  • Zhang M  et al. HDAC6 Regulates the MRTF-A/SRF Axis and Vascular Smooth Muscle Cell Plasticity. JACC Basic Transl Sci 3:782-795 (2018). Read more (PubMed: 30623138) »
See all 48 Publications for this product

Customer reviews and Q&As

1-9 of 9 Q&A

Answer


Myofibroblasts are halfway between differentiation from fibroblasts into smooth muscle cells. Thus, certain markers expressed in SMC could be expected to be expressed in myofibroblasts as well. The paper by Jones et al shows the antibody detects myofibroblasts and SMCs. Given this, I believe that this antibody is not suitable for your specific needs.

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Question
Answer

DISCOUNT CODE: ***
Expiration date: December 17, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab53219with human samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for contacting us.

Your credit note IDs for the refundare xxx (2 vials) and xxx (1 vial).

I am sorry that this antibody had a mistake on the datasheet - which we have corrected in the meantime. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for your email.

As for ab681, I have contacted the lab for more information, and will let you know what I hear back.


For ab683, we know that this antibody reacts with human, rabbit, and rat smooth muscle myosin heavy chain 11 (SM2). Does not cross-react with smooth muscle Myosin heavy chain 1.


As for ab53219, the antibody was raised against bovine tracheal smooth muscle myosin, which is the myosin as it is found in bovine trachea. Specificity for subunit 1 or 2 has not been determined.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Regretably, this mistake was caused by naming the product incorrectly. Instead "11", it had been written as "II" in oursystem which then got somehow converted in a "2".

The database links on the datasheet were still correct as well as the information in the relevance section. But, unfortunately, the product was called "smooth muscle myosin heavy chain 2" instead "smooth muscle myosin heavy chain 11" - in several places of the datasheet. We are very sorry about this mistake, and have corrected it in the meantime.

This mistake occurred for all 3 "smooth muscle myosin heavy chain 11" antibodies we have in the catalog: ab53219, ab683, and ab82541.

Any reference to myosin 2 on these datasheets have already been passed on for update, and should be corrected very soon.

We currently only have 7 antibodies to "Myosin, smooth muscle heavy chain 1 and 2 " in our catalog.

Please let me know how you would like to be compensated for our mistake. The options would be a free of charge replacement, credit or a refund.

I again sincerely apologize for this oversight, and look forward to hear back from you toresolve this case for you.

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Question
Answer

Thank you for your reply. We sincerely apologize for this mistake on the datasheet and for the inconvenience this has caused.
As you requested, I have issued a free of charge replacement with the order number xxx with ab6046.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thank you for your email.
Could you please let me know how you would like to be compensated?
The options are a free of charge replacement with an antibody of your choice, a credit or a refund.
I look forward to hear back from you and resolve this for you.

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Answer

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that there was a mistake on our datasheet for this antibody. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for contacting us. I have found your order in our system (Abcam order number xxx). According to your request, I have issued you a credit.

Your credit note ID is xxx.

I am very sorry about the mistake we madeon the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

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