Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)
Key features and details
- Rabbit polyclonal to smooth muscle Myosin heavy chain 11
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-smooth muscle Myosin heavy chain 11 antibody
See all smooth muscle Myosin heavy chain 11 primary antibodies -
Description
Rabbit polyclonal to smooth muscle Myosin heavy chain 11 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 82% homology with non-muscle myosin 9. The cross-reactivity with this protein has not been confirmed experimentally.
Replenishment batches of our polyclonal antibody, ab82541 are tested in WB. Previous batches were additionally validated in ICC. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab124679.
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Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93494) -
Positive control
- WB: Bladder tissue; Heart tissue; PC3 whole cell; HUVEC whole cell. ICC: SV40LT-SMC cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa).
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ICC |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa). |
ICC
Use a concentration of 1 µg/ml. |
Target
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Function
Muscle contraction. -
Tissue specificity
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. -
Involvement in disease
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 530050 Cow
- Entrez Gene: 4629 Human
- Entrez Gene: 17880 Mouse
- Entrez Gene: 100009145 Rabbit
- Entrez Gene: 24582 Rat
- Omim: 160745 Human
- SwissProt: P35749 Human
- SwissProt: O08638 Mouse
see all -
Alternative names
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
Images
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ab82541 staining smooth muscle Myosin heavy chain 11 in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab82541 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1 µg/ml
Lane 1 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 227 kDa
Observed band size: 227 kDa
Additional bands at: 49 kDa, 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1/2000 dilution + whole cell lysate prepared from human PASMC, P6 at 300 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 227 kDa
Observed band size: 227 kDa
Additional bands at: 100 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab82541 has been referenced in 8 publications.
- Zakharova I et al. Mitomycin-Treated Endothelial and Smooth Muscle Cells Suitable for Safe Tissue Engineering Approaches. Front Bioeng Biotechnol 10:772981 (2022). PubMed: 35360387
- Li Z et al. miR-25-5p regulates endothelial progenitor cell differentiation in response to shear stress through targeting ABCA1. Cell Biol Int 45:1876-1886 (2021). PubMed: 33945659
- Tao W et al. MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm. J Cell Mol Med 25:6056-69 (2021). PubMed: 34132029
- Goikuria H et al. Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition. Cells 7:N/A (2018). PubMed: 29562638
- Spray S et al. Alterations in the Cerebral Microvascular Proteome Expression Profile After Transient Global Cerebral Ischemia in Rat. J Mol Neurosci 61:396-411 (2017). PubMed: 27933490
- Alloza I et al. RNAseq based transcriptomics study of SMCs from carotid atherosclerotic plaque: BMP2 and IDs proteins are crucial regulators of plaque stability. Sci Rep 7:3470 (2017). WB ; Human . PubMed: 28615715
- Zakharova IS et al. Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts. J Transl Med 15:54 (2017). PubMed: 28257636
- Lian X et al. Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling. Stem Cell Reports 3:804-16 (2014). ICC/IF ; Human . PubMed: 25418725