The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa).
Use a concentration of 1 µg/ml.
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
Involvement in disease
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
Contains 1 IQ domain. Contains 1 myosin head-like domain.
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
ICC/IF image of ab82541 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82541, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)
All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1 µg/ml
Lane 1 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654) Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 227 kDa Observed band size: 227 kDa Additional bands at: 49 kDa, 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)Image courtesy of an anonymous Abreview.
Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1/2000 dilution + whole cell lysate prepared from human PASMC, P6 at 300 µg
Secondary HRP conjugated goat anti-rabbit polyclonal at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 227 kDa Observed band size: 227 kDa Additional bands at: 100 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Alloza I et al. RNAseq based transcriptomics study of SMCs from carotid atherosclerotic plaque: BMP2 and IDs proteins are crucial regulators of plaque stability. Sci Rep7:3470 (2017).
Read more (PubMed: 28615715) »