Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade
See all smooth muscle Myosin heavy chain 11 primary antibodies -
Description
Rabbit polyclonal to smooth muscle Myosin heavy chain 11 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: CHIPseq, WB, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human smooth muscle Myosin heavy chain 11 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (2 peptides used, aa 50 - 100)
Database link: P35749 -
Positive control
- Chromatin prepared from ME-1 cells; ME-1 nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab195481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| CHIPseq | Use at an assay dependent concentration. Use 5 µl per ChIP using chromatin prepared from 1.5 million cells. |
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| WB | 1/1000. Predicted molecular weight: 227 kDa. | |
| ChIP | Use at an assay dependent concentration. Use 5 µl per ChIP using chromatin prepared from 1.5 million cells. |
Target
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Function
Muscle contraction. -
Tissue specificity
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. -
Involvement in disease
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 4629 Human
- Omim: 160745 Human
- SwissProt: P35749 Human
- Unigene: 460109 Human
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Alternative names
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
Images
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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)Lane 1 : CBFb antibody at 1/1000 dilution
Lane 2 : Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481) at 1/1000 dilution
All lanes : ME-1 nuclear extract
Predicted band size: 227 kDaThe antibody was diluted in TBS-Tween containing 5% skimmed milk.
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ChIP - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)ChIP results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11.
ChIP assays were performed using ME-1 cells, ab195481 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 µl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
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CHIPseq - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)ChIPseq results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11.
ChIP was performed. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.
Protocols
Datasheets and documents
References
ab195481 has not yet been referenced specifically in any publications.
