Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description

    Rabbit polyclonal to smooth muscle Myosin heavy chain 11 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: CHIPseq, WB, ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human smooth muscle Myosin heavy chain 11 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (2 peptides used, aa 50 - 100)
    Database link: P35749

  • Positive control

    • Chromatin prepared from ME-1 cells; ME-1 nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab195481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
CHIPseq Use at an assay dependent concentration.

Use 5 µl per ChIP using chromatin prepared from 1.5 million cells.

WB 1/1000. Predicted molecular weight: 227 kDa.
ChIP Use at an assay dependent concentration.

Use 5 µl per ChIP using chromatin prepared from 1.5 million cells.

Target

  • Function

    Muscle contraction.
  • Tissue specificity

    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease

    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • Lane 1 : CBFb antibody at 1/1000 dilution
    Lane 2 : Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481) at 1/1000 dilution

    All lanes : ME-1 nuclear extract

    Predicted band size: 227 kDa



    The antibody was diluted in TBS-Tween containing 5% skimmed milk.

  • ChIP results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11.

    ChIP assays were performed using ME-1 cells, ab195481 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 µl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

  • ChIPseq results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11. 

    ChIP was performed. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

References

ab195481 has not yet been referenced specifically in any publications.

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