RecombinantRabMAb

Recombinant Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11
  • Suitable for: ICC, Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]
    See all smooth muscle Myosin heavy chain 11 primary antibodies

  • Description

    Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human ovary and prostate tissues; WB: Human bladder, artery and testis tissue lysates; Flow Cyt (intra): 293 cells; ICC: Human smooth muscle and A673 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/50.
Flow Cyt (Intra)
1/20.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 227 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250
Notes
ICC
1/50.
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 227 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250

Target

  • Function

    Muscle contraction.

  • Tissue specificity

    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.

  • Involvement in disease

    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.

  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • Cellular localization

    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt

  • Database links

    • Alternative names

      • AAT4 antibody
      • DKFZp686D10126 antibody
      • DKFZp686D19237 antibody
      • FAA4 antibody
      • FLJ35232 antibody
      • MGC126726 antibody
      • MGC32963 antibody
      • MYH 11 antibody
      • MYH11 antibody
      • MYH11_HUMAN antibody
      • Myosin 11 antibody
      • Myosin heavy chain 11 antibody
      • Myosin heavy chain 11 smooth muscle antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain smooth muscle isoform antibody
      • Myosin heavy polypeptide 11 smooth muscle antibody
      • Myosin-11 antibody
      • SMHC antibody
      • SMMHC antibody
      • smooth muscle isoform antibody
      • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
      • Smooth muscle myosin heavy chain isoform SM2 antibody
      see all

    Images

    • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/5000 dilution (purified) + Human testis lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 227 kDa
      Observed band size: 227 kDa

    • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunocytochemistry/ Immunofluorescence analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/50 dilution (2.34 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/1000 dilution (0.12 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/1000 dilution (unpurified)

      Lane 1 : Human bladder lysate
      Lane 2 : Human artery lysate
      Lane 3 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 227 kDa

    • Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Intracellular Flow Cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)This image is courtesy of an anonymous abreview.

      unpurified ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

      See Abreview

    • Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    References (7)

    Publishing research using ab133567? Please let us know so that we can cite the reference in this datasheet.

    ab133567 has been referenced in 7 publications.

    • Xu Z  et al. Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca2+ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia. Exp Ther Med 21:322 (2021). PubMed: 33732295
    • Wang Y  et al. Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response. Cell Discov 7:36 (2021). PubMed: 34035226
    • Ma Q  et al. MicroRNA-29b targeting of cell division cycle 7-related protein kinase (CDC7) regulated vascular smooth muscle cell (VSMC) proliferation and migration. Ann Transl Med 8:1496 (2020). PubMed: 33313241
    • Okada H  et al. Phenotype and physiological significance of the endocardial smooth muscle cells in human failing hearts. Circ Heart Fail 8:149-55 (2015). IHC-P ; Human . PubMed: 25466765
    • Pei H  et al. Overexpression of MicroRNA-145 Promotes Ascending Aortic Aneurysm Media Remodeling through TGF-ß1. Eur J Vasc Endovasc Surg 49:52-9 (2015). PubMed: 25465469
    • Pereira L  et al. Intrauterine growth restriction caused by underlying congenital cytomegalovirus infection. J Infect Dis 209:1573-84 (2014). Human . PubMed: 24403553
    • Wang RJ  et al. Down-regulated MYH11 expression correlates with poor prognosis in stage II and III colorectal cancer. Asian Pac J Cancer Prev 15:7223-8 (2014). PubMed: 25227818

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung, Skeletal Muscle, Heart and Aorta)
    Gel Running Conditions
    Reduced Denaturing (5% Tris-glycine)
    Loading amount
    10 µg
    Specification
    Lung, Skeletal Muscle, Heart and Aorta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    DR. Rebecca Deaton

    Verified customer

    Submitted Mar 19 2019

    Immunocytochemistry/ Immunofluorescence

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    1%serum,0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Sample
    Human Cell (smooth muscle cells)
    Specification
    smooth muscle cells
    Permeabilization
    Yes - see blocking buffer
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 07 2013

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