Recombinant
RabMAb

Recombinant Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

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Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]
    See all smooth muscle Myosin heavy chain 11 primary antibodies

  • Description

    Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human smooth muscle Myosin heavy chain 11 aa 1950 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human ovary and prostate tissues; Human bladder, artery and testis lysates. FC: 293 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 227 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Muscle contraction.

    • Tissue specificity

      Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.

    • Involvement in disease

      Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
      Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.

    • Sequence similarities

      Contains 1 IQ domain.
      Contains 1 myosin head-like domain.

    • Domain

      The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    • Cellular localization

      Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
    • Information by UniProt

    • Database links

      • Alternative names

        • AAT4 antibody
        • DKFZp686D10126 antibody
        • DKFZp686D19237 antibody
        • FAA4 antibody
        • FLJ35232 antibody
        • MGC126726 antibody
        • MGC32963 antibody
        • MYH 11 antibody
        • MYH11 antibody
        • MYH11_HUMAN antibody
        • Myosin 11 antibody
        • Myosin heavy chain 11 antibody
        • Myosin heavy chain 11 smooth muscle antibody
        • Myosin heavy chain antibody
        • Myosin heavy chain smooth muscle isoform antibody
        • Myosin heavy polypeptide 11 smooth muscle antibody
        • Myosin-11 antibody
        • SMHC antibody
        • SMMHC antibody
        • smooth muscle isoform antibody
        • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
        • Smooth muscle myosin heavy chain isoform SM2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

        Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with ab133567 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/1000 dilution

        Lane 1 : Human bladder lysate
        Lane 2 : Human artery lysate
        Lane 3 : Human testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 227 kDa

      • Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

        Flow cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with ab133567 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
        Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)This image is courtesy of an anonymous abreview.

        ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

        See Abreview

      References

      This product has been referenced in:

      • Okada H  et al. Phenotype and physiological significance of the endocardial smooth muscle cells in human failing hearts. Circ Heart Fail 8:149-55 (2015). IHC-P ; Human . Read more (PubMed: 25466765) »
      • Pei H  et al. Overexpression of MicroRNA-145 Promotes Ascending Aortic Aneurysm Media Remodeling through TGF-ß1. Eur J Vasc Endovasc Surg 49:52-9 (2015). Read more (PubMed: 25465469) »
      See all 3 Publications for this product

      Publishing research using ab133567? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Lung, Skeletal Muscle, Heart and Aorta)
      Gel Running Conditions
      Reduced Denaturing (5% Tris-glycine)
      Loading amount
      10 µg
      Specification
      Lung, Skeletal Muscle, Heart and Aorta
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Dr. Rebecca Deaton

      Verified customer

      Submitted Mar 19 2019

      Immunocytochemistry/ Immunofluorescence

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      1%serum,0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
      Sample
      Human Cell (smooth muscle cells)
      Specification
      smooth muscle cells
      Permeabilization
      Yes - see blocking buffer
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 07 2013

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