Recombinant Alexa Fluor® 488 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab197524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]
See all smooth muscle Myosin heavy chain 11 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: normal human kidney and normal human colon tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5336(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
- Alexa Fluor® 647 Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab196982)
- HRP Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab196983)
- Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Muscle contraction. -
Tissue specificity
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. -
Involvement in disease
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 4629 Human
- Omim: 160745 Human
- SwissProt: P35749 Human
- Unigene: 460109 Human
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Alternative names
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
Images
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IHC image of smooth muscle Myosin heavy chain 11 in a section of formalin-fixed, paraffin-embedded, normal human colon. The section was pre-treated using pressure cooker heat mediated antigen retrieval with Tris-EDTA (ph9.0) and incubated overnight at +4°C with ab197524 at 1/250 dilution. The section was counterstained with DAPI and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of smooth muscle Myosin heavy chain 11 in a section of formalin-fixed, paraffin-embedded, normal human kidney. The section was pre-treated using pressure cooker heat mediated antigen retrieval with Tris-EDTA (ph9.0) and incubated overnight at +4°C with ab197524 at 1/250 dilution. The section was counterstained with DAPI and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab197524 has been referenced in 1 publication.
- Hendrickx JO et al. Altered stress hormone levels affect in vivo vascular function in the hAPP23+/- overexpressing mouse model of Alzheimer's disease. Am J Physiol Heart Circ Physiol 321:H905-H919 (2021). PubMed: 34506227