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    smooth-muscle-myosin-heavy-chain-11-antibody-sp314-n-terminal-ab224804.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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RecombinantRabMAb

Recombinant Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

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  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP314] to smooth muscle Myosin heavy chain 11 - N-terminal
  • Suitable for: IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description

    Rabbit monoclonal [SP314] to smooth muscle Myosin heavy chain 11 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human small intestine, colon, prostate, fallopian tube and ovary tissues, rat and mouse colon tissue. IHC-Fr: Mouse and rat small intestine tissue. Flow Cyt: C2C12 cells
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP314
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Myosins
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Alternative Versions

    • Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - BSA and Azide free (ab240983)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/500.
Flow Cyt
1/80.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Notes
IHC-Fr
1/500.
Flow Cyt
1/80.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Target

  • Function

    Muscle contraction.
  • Tissue specificity

    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease

    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Target information above from: UniProt accession P35749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4629 Human
    • Entrez Gene: 17880 Mouse
    • Entrez Gene: 24582 Rat
    • Omim: 160745 Human
    • SwissProt: P35749 Human
    • SwissProt: O08638 Mouse
    • SwissProt: Q63862 Rat
    • Unigene: 460109 Human
    • Unigene: 250705 Mouse
    • Unigene: 94969 Rat
    see all
  • Alternative names

    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in rat colon, performed on a Leica Biosystems BOND™ RX instrument. 
    The section was incubated with ab224804 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/400 dilution (0.27 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in mouse colon, performed on a Leica Biosystems BOND™ RX instrument. 
    The section was incubated with ab224804 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224804 for 10 mins at room temperature.

  • Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Immunohistochemistry (Frozen) analysis of mouse small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Flow cytometry analysis of C2C12 (Mouse myoblasts myoblast) labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/80 dilution (1.12 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Formalin-fixed, paraffin-embedded human colon tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Immunohistochemistry (Frozen) analysis of rat small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Formalin-fixed, paraffin-embedded human small intestine tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal (ab224804)

    Formalin-fixed, paraffin-embedded human ovary tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab224804? Please let us know so that we can cite the reference in this datasheet.

ab224804 has been referenced in 1 publication.

  • Liu B  et al. Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes. Stem Cells Int 2021:8859071 (2021). PubMed: 33897781

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Cultured Primary Pig Smooth Muscle Cells)
Permeabilization
Yes - 0.5% Triton X-100 in PBST
Specification
Cultured Primary Pig Smooth Muscle Cells
Blocking step
Serum as blocking agent for 50 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Luke Batty

Verified customer

Submitted May 07 2021

Western blot abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [SP314] - N-terminal

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (A10 smooth muscle cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Treatment
Hypoxia 24 hours 2% O2
Specification
A10 smooth muscle cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Serjei Mirkulov

Verified customer

Submitted Apr 21 2021

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