Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1] (ab233939)
Key features and details
- Mouse monoclonal [SMMS-1] to smooth muscle Myosin heavy chain I
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-smooth muscle Myosin heavy chain I antibody [SMMS-1]
See all smooth muscle Myosin heavy chain I primary antibodies -
Description
Mouse monoclonal [SMMS-1] to smooth muscle Myosin heavy chain I -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Pig -
Immunogen
Tissue, cells or virus corresponding to Human smooth muscle Myosin heavy chain I. (Human uterus extract).
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Positive control
- IHC-P: Human breast carcinoma and leiomyosarcoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
SMMS-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Muscle contraction. -
Tissue specificity
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles. -
Involvement in disease
A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Aortic aneurysm, familial thoracic 4 -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Contains 1 IQ domain.
Contains 1 myosin motor domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 101097693 Cat
- Entrez Gene: 396211 Chicken
- Entrez Gene: 530050 Cow
- Entrez Gene: 479836 Dog
- Entrez Gene: 100714060 Guinea pig
- Entrez Gene: 4629 Human
- Entrez Gene: 100009145 Rabbit
- Entrez Gene: 24582 Rat
see all -
Alternative names
- FAA4 antibody
- MYH11 antibody
- MYH11_HUMAN antibody
see all
Images
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for smooth muscle Myosin heavy chain I using ab233939 at 1 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human leiomyosarcoma tissue stained for smooth muscle Myosin heavy chain I using ab233939 at 1 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233939 has not yet been referenced specifically in any publications.