Key features and details
- Rabbit polyclonal to Smoothelin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Smoothelin antibody
See all Smoothelin primary antibodies
DescriptionRabbit polyclonal to Smoothelin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human prostate tissue; WB: PC-3 and HeLa cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.|
|IHC-P||1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionStructural protein of the cytoskeleton.
Tissue specificitySmooth muscle; contractile or vascular (for the long form).
Sequence similaritiesBelongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
Cellular localizationCytoplasm > cytoskeleton. Exhibits a filamentous organization.
- Information by UniProt
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Smoothelin with ab204305 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-Smoothelin antibody (ab204305) at 0.4 µg/ml
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 99 kDa
Loading control: Anti-PPIB
ab204305 has been referenced in 2 publications.
- Domke LM & Franke WW The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res N/A:N/A (2018). PubMed: 30591979
- Yang Q et al. Transplantation of Human Urine-Derived Stem Cells Transfected with Pigment Epithelium-Derived Factor to Protect Erectile Function in a Rat Model of Cavernous Nerve Injury. Cell Transplant 25:1987-2001 (2016). PubMed: 27075964