Overview

  • Product name

  • Description

    Rabbit polyclonal to Smoothelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Smoothelin aa 6-100.
    Sequence:

    LAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEEALASKRFR AERQDNKENWLHSQQREAEQRAALARLAGQLESMNDVEELTALLR


    Database link: P53814

  • Positive control

    • Human smooth muscle tissue; RT-4 and U-251 MG cell lysates; U-2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab204305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Predicted molecular weight: 99 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling Smoothelin with ab204305 at a 1/2500 dilution.

  • All lanes : Anti-Smoothelin antibody (ab204305) at 1/1000 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Developed using the ECL technique.

    Predicted band size: 99 kDa

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling Smoothelin with ab204305 at 4 µg/ml (green).

References

This product has been referenced in:

  • Domke LM & Franke WW The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res N/A:N/A (2018). Read more (PubMed: 30591979) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (smooth muscle cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Treatment
1 uM MEKi
Specification
smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 29 2019

Application
Immunocytochemistry
Sample
Human Cultured Cells (human aortic smooth muscle cell)
Permeabilization
Yes - 0.1% Triton X-100
Specification
human aortic smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 04 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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