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Our Abpromise guarantee covers the use of ab204603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 99 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
Immunohistochemical analysis of formalin fixed, paraffin embedded Human smooth muscle tissue labeling Smoothelin with ab204603 at 1/1000.
Immunofluorescent analysis of paraformaldehyde fixed, Triton X-100 permeabilized A431 cells labeling Smoothelin with ab204603 at 4µg/ml (green).
ab204603 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"