Key features and details
- Rabbit polyclonal to Smoothelin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Smoothelin antibody
See all Smoothelin primary antibodies
DescriptionRabbit polyclonal to Smoothelin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan
Synthetic peptide corresponding to Human Smoothelin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).|
FunctionStructural protein of the cytoskeleton.
Tissue specificitySmooth muscle; contractile or vascular (for the long form).
Sequence similaritiesBelongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
Cellular localizationCytoplasm > cytoskeleton. Exhibits a filamentous organization.
- Information by UniProt
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100,95 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).
ab74379 has not yet been referenced specifically in any publications.