Product nameAnti-Smoothelin antibody
See all Smoothelin primary antibodies
DescriptionRabbit polyclonal to Smoothelin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan
Synthetic peptide corresponding to Human Smoothelin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab74379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human Smoothelin peptide (ab74378).|
FunctionStructural protein of the cytoskeleton.
Tissue specificitySmooth muscle; contractile or vascular (for the long form).
Sequence similaritiesBelongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
Cellular localizationCytoplasm > cytoskeleton. Exhibits a filamentous organization.
- Information by UniProt
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100,95 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).
ab74379 has not yet been referenced specifically in any publications.