Overview

  • Product name

  • Description

    Rabbit polyclonal to Smoothelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Smoothelin aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab74378)

  • Positive control

    • This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab74379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human Smoothelin peptide (ab74378).

Target

Images

  • All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 100,95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).

References

ab74379 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your interest in our products.

Discount code: XXXXX

Expiration date: 02-March-2013

I am very pleased to hear you would like to test ab74379 with Mouse species. This code will give you 370€ off your next order before the expiration date. To redeem this offer, please submit an Abreview for this antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

DISCOUNT CODE: xxxxxxx
Expiration date: 19th of August 2012

I am very pleased to hear you would like to accept our offer and test ab74379 inICC with mouse samples. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

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Answer

Thank you for contacting us in regards to using anti-Smoothelin antibody (ab69724 or ab74379) for ICC of mouse samples.

As discussed over the phone, unfortunately, whilst ab69724 has not been used with mouse samples as yet, ab74379 has only been validated in western blotting. We can therefore not guarantee that either antibody would work with the experiments you are planning to perform. I would however say that unfortunately ab69724 is not very likely to be able to detect the mouse protein as the immunogen used to raise that antibody (GSPEPPPSPPKTTSPEPQES) does not share significanthomology with the mouse protein (please refer to the attached document). I would therefore say that ab74379, which has been shown to work with the mouse samples would be more worthwhile trying.

If you would like to purchase ab74379 for your experiments we can offer you a testing discount. This offer means that if you test the antibody in ICC with mouse species and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint in the offer is that the antibody to be tested needs to be purchased, tested, the abreview submitted and the "free" antibody claimed within 4 months. If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of either ab74379.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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