Overview

  • Product name
    Anti-Smoothelin antibody [R4A]
    See all Smoothelin primary antibodies
  • Description
    Mouse monoclonal [R4A] to Smoothelin
  • Host species
    Mouse
  • Specificity
    Reacts with the 59kD and 100kD protein, corresponding to smoothelin A and B, respectively.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC, WBmore details
  • Species reactivity
    Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
    Does not react with: Mouse, Rat, Hamster
  • Immunogen

    Other Immunogen Type corresponding to Chicken Smoothelin. Cytoskeletal extract of chicken gizzard.

Properties

Applications

Our Abpromise guarantee covers the use of ab21108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/200.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB 1/100 - 1/200. Predicted molecular weight: 102 kDa.

Target

  • Function
    Structural protein of the cytoskeleton.
  • Tissue specificity
    Smooth muscle; contractile or vascular (for the long form).
  • Sequence similarities
    Belongs to the smoothelin family.
    Contains 1 CH (calponin-homology) domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35168 antibody
    • FLJ35365 antibody
    • FLJ35620 antibody
    • FLJ38597 antibody
    • Smoothelin antibody
    • SMSMO antibody
    • Smtn antibody
    • SMTN_HUMAN antibody
    see all

Images

  • Paraffin-embedded human smooth muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin section of human muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.

  • ab21108 staining human small intestine tissue sections by IHC-P.  The sample was formaldehyde fixed and subjected to a heat mediated antigen retrieval in EDTA prior to incubation with ab21108 (1/1600) for 16 minutes at 25°C.  Polymer detection was with DAB (no enhancement) and a hematoxylin counterstain.

    See Abreview

References

This product has been referenced in:
  • Salem SA  et al. Human adipose tissue derived stem cells as a source of smooth muscle cells in the regeneration of muscular layer of urinary bladder wall. Malays J Med Sci 20:80-7 (2013). Read more (PubMed: 24044001) »
  • Bockmeyer CL  et al. Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis. Nephrol Dial Transplant 27:3493-501 (2012). Read more (PubMed: 22319217) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your call today.
I apologize for the inconvenience with ab683, and as we discussed I would be happy to send a replacement or issue a credit or refund. Also, please keep me updated about any further information about ab21108.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (small intestine)
Specification
small intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Username

Mr. Ronald Houston

Verified customer

Submitted Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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