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Other Immunogen Type corresponding to Chicken Smoothelin. Cytoskeletal extract of chicken gizzard.
Our Abpromise guarantee covers the use of ab21108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/200.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|WB||1/100 - 1/200. Predicted molecular weight: 102 kDa.|
Paraffin-embedded human smooth muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.
Paraffin section of human muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.
ab21108 staining human small intestine tissue sections by IHC-P. The sample was formaldehyde fixed and subjected to a heat mediated antigen retrieval in EDTA prior to incubation with ab21108 (1/1600) for 16 minutes at 25°C. Polymer detection was with DAB (no enhancement) and a hematoxylin counterstain.
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