Overview

  • Product name
    Anti-Smoothelin antibody [R4A]
    See all Smoothelin primary antibodies
  • Description
    Mouse monoclonal [R4A] to Smoothelin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, ICC, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
    Does not react with: Mouse, Rat, Hamster
  • Immunogen

    Cytoskeletal extract of chicken gizzard.

Properties

Applications

Our Abpromise guarantee covers the use of ab8969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/1000.

Target

  • Function
    Structural protein of the cytoskeleton.
  • Tissue specificity
    Smooth muscle; contractile or vascular (for the long form).
  • Sequence similarities
    Belongs to the smoothelin family.
    Contains 1 CH (calponin-homology) domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35168 antibody
    • FLJ35365 antibody
    • FLJ35620 antibody
    • FLJ38597 antibody
    • Smoothelin antibody
    • SMSMO antibody
    • Smtn antibody
    • SMTN_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.

  • Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.

  • Immunoblotting of human colon smooth muscle tissue incubated with (a) monoclonal antibody R4A (ab8969) to smoothelin (a), and subsequently incubated with the monoclonal desmin antibody RD301.

    Immunoblotting of human colon smooth muscle tissue incubated with (a) monoclonal antibody R4A (ab8969) to smoothelin (a), and subsequently incubated with the monoclonal desmin antibody RD301.
  • Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

  • ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.

    See Abreview

  • Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

References

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • Timraz SB  et al. In-depth evaluation of commercially available human vascular smooth muscle cells phenotype: Implications for vascular tissue engineering. Exp Cell Res 343:168-76 (2016). ICC/IF ; Human . Read more (PubMed: 27079869) »
See all 16 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cells)
Specification
Vascular smooth muscle cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton X-100
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C

Mr. G Krenning

Verified customer

Submitted Sep 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (aortic smooth muscle cells)
Loading amount
10 µg
Specification
aortic smooth muscle cells
Gel Running Conditions
Reduced Denaturing (Tris-acetate 3-8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue lysate - other (canine myocardial infarct)
Specification
canine myocardial infarct
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2%

Dr. Marcin Dobaczewski

Verified customer

Submitted Aug 11 2005

Answer

Thank you for your enquiry and your interest in our products. We would like to ask you please to provide some more information about your IHC staining and fill in this specific IHC Questionnaire. Your answer will help us identify the source of the problem and give you sufficient technical support. 1. Order details: • Batch number: • Abcam order or Purchase order number: • Antibody storage conditions (temperature/reconstitution etc) 2. Please describe the problem (high background, no staining etc). 3. On what material are you testing the antibody in IHC • Species: • Cell Iine or tissue: 4. How did you fix the samples? • Ethanol, methanol: (was it ice cold) • Acetone: (was it ice cold) • Paraformaldehyde (percentage, perfusion fixed on not): • Fixation time: • Fixation temperature: 5. For formalin-fixed paraffin embedded tissue: did you apply antigen retrieval step? • Enzymatic method: • Heat mediated technique: • Other : 6. Blocking steps: • For HRP detection method: did you block endogenous peroxidases: • How did you block the unspecific binding sites: serum, BSA, Milk, other • For how long: 7. Primary antibody • Specification (in which species was it raised against): • At what dilution(s) have you tested this antibody: • What dilution buffer was used: • Incubation time: • Incubation temperature: • What washing steps were done: 8. Secondary antibody • Specification (in which species was it raised against): • What dilution buffer was used: • Incubation time: • Incubation temperature: • What washing steps were done: • Do you know whether the problems you are experiencing come from the secondary? 9. What detection method are you using? 10. Background staining • Please provide an image of your staining 11. Which detection system did you use? 12. Did you apply positive and negative controls along with the samples? Please specify. 13. Optimization attempts • How many times have you tried the IHC? • Do you obtain the same results every time? • What steps have you altered? We look forward to hearing from you soon.

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Answer

Ab8969 is in an unpurified form (tissue culture supernatant) and we cannot guarantee the concentrations for unpurified material. When we know what the recommended dilutions are for an antibody we will state them on the datasheet, otherwise the dilutions are assay dependent.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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