Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)This image is courtesy of an anonymous Abreview
ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.
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Timraz SB et al. In-depth evaluation of commercially available human vascular smooth muscle cells phenotype: Implications for vascular tissue engineering. Exp Cell Res343:168-76 (2016).
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