Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab58574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 86 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionG protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
- Information by UniProt
- Frizzled family member 11 antibody
- FZD11 antibody
- Gx antibody
Anti-Smoothened antibody (ab58574) at 0.3 µg/ml + Human Bone Marrow lysate at 35 µg
Predicted band size: 86 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab58574 has not yet been referenced specifically in any publications.