• Product name
  • Description
    Rabbit polyclonal to Smt3
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant protein (S. cerevisiae).

  • Positive control
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.
  • General notes
    Centrifuge product if not completely clear after standing at room temperature.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab14405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
ELISA 1/1000 - 1/5000.


  • Function
    Not known; suppressor of MIF2 mutations.
  • Sequence similarities
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Information by UniProt
  • Database links
    • Alternative names
      • DmSUMO 1 antibody
      • Small Ubiquitin-like modifier antibody
      • SMT3 antibody
      • SMT3_YEAST antibody
      • Ubiquitin like protein of the SUMO family antibody
      • Ubiquitin like protein SMT3 antibody
      • Ubiquitin-like protein SMT3 antibody
      see all


    • All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution

      Lane 1 : 3 : 5 : 7 : 9 : 11 : 13, Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
      Lane 2 : 4 : 6 : 8 : 10 : 12 : 14, Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS

      Lysates/proteins at 2 µg per lane.
    • ab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.

      See Abreview


    This product has been referenced in:
    • Bauer SL  et al. Helicase/SUMO-targeted ubiquitin ligase Uls1 interacts with the Holliday junction resolvase Yen1. PLoS One 14:e0214102 (2019). Read more (PubMed: 30897139) »
    • Ainscough JS  et al. Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36?. Proc Natl Acad Sci U S A 114:E2748-E2757 (2017). Read more (PubMed: 28289191) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A


    The immunogen is full-length protein, untagged, and the amino acid sequence is given at the UniProt entry for S. cerevisiae Smt3,

    http://www.uniprot.org/uniprot/Q12306 .

    This antibody has not been tested by immunprecipitation, or by immobilization on beads.

    Read More
    Western blot
    Saccharomyces cerevisiae Cell lysate - whole cell (log phase yeast cells)
    Loading amount
    100 µg
    log phase yeast cells
    Gel Running Conditions
    Reduced Denaturing (6-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C

    Dr. O Kerscher

    Verified customer

    Submitted Nov 10 2011

    Escherichia coli Recombinant protein (BL21(DE3))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 28 2010

    Western blot
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Apr 26 2010


    Thank you for your enquiry. I am pleased that you are happy with the performance of Smt3 antibody (ab14405). Please can I encourage you to leave an Abreview. This can be done by clicking on the "Abreview" tab on the product datasheet. In return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Unfortunately we do not have a non-immunized rabbit IgG control antibody, or pre-immune serum for this rabbit. Unfortunately I cannot offer you an immunoglobulin isotype control either given that this antibody is likely to be comprised of a mixture of many different IgG sub class. I would recommend that you use a no antibody control, performed in parallel with this antibody. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More


    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please rest assured that ab14405 is fully characterized and is guaranteed to work in Western blot with S. cerevisiae samples. If you have any additional questions or concerns, please contact us again.

    Read More


    Thank you for your enquiry. Ab5211 is a Fast-Track antibody and has not yet been fully characterized, therefore we cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide. We do have some preliminary Western blot results, the image can be seen on the online datasheet. I urge you to submit your results to us; we will award 1,000 Abcam points to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 1,000 Abcam points. Other useful positive and negative feedback will be rewarded with 50 Abcam points. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com Ab14405 has been fully characterized. The antibody cross-reacts with S. cerevisiae and has been tested for application in Western blotting and ELISA. Since this antibody has been fully characterized, we guarantee it to work as we state on the online datasheet. If you purchase it and have difficulty, we will first trouble-shoot and then can offer a refund or a free of charge replacement. If you have any additional questions, please contact us again.

    Read More

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