Anti-SMUG1 antibody (ab15716)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-SMUG1 antibody
See all SMUG1 primary antibodies -
DescriptionGoat polyclonal to SMUG1
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Host speciesGoat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide: PQAFLLGSIHEPA, corresponding to N terminal amino acids 2-14 of SMUG1.
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Positive control
- Molt-4 lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab15716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human SMUG1 peptide (ab23269). |
Target
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FunctionRecognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 23583 Human
- Omim: 607753 Human
- SwissProt: Q53HV7 Human
- Unigene: 632721 Human
- Unigene: 731659 Human
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Alternative names
- FDG antibody
- HMUDG antibody
- MGC104370 antibody
see all
Images
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Western blot - Anti-SMUG1 antibody (ab15716)ab15716 at 0.5µ
Datasheets and documents
Protocols
References
This product has been referenced in:
- Eldin P et al. Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA. Nucleic Acids Res N/A:N/A (2013). Read more (PubMed: 24178031) »
- Zaika E et al. p73 protein regulates DNA damage repair. FASEB J : (2011). WB ; Human . Read more (PubMed: 21891782) »
