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    smug1-antibody-ab15716.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Base Excision Repair
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Anti-SMUG1 antibody (ab15716)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Flow Cytometry - Anti-SMUG1 antibody (ab15716)
  • Immunocytochemistry - Anti-SMUG1 antibody (ab15716)
  • Immunocytochemistry - Anti-SMUG1 antibody (ab15716)

Key features and details

  • Goat polyclonal to SMUG1
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SMUG1 protein (ab132162)
Secondary
Product image
Donkey Anti-Goat IgG H&L (HRP) (ab97110)

View more associated products

Overview

  • Product name

    Anti-SMUG1 antibody
    See all SMUG1 primary antibodies
  • Description

    Goat polyclonal to SMUG1
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide: PQAFLLGSIHEPA, corresponding to N terminal amino acids 2-14 of SMUG1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC: U2OS and MCF7 cells; Flow Cyt: MCF7 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human SMUG1 protein (ab132162)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
Application Abreviews Notes
Flow Cyt
Use a concentration of 10 µg/ml.
ICC
Use a concentration of 10 µg/ml.
Notes
Flow Cyt
Use a concentration of 10 µg/ml.
ICC
Use a concentration of 10 µg/ml.

Target

  • Function

    Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q53HV7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23583 Human
    • Omim: 607753 Human
    • SwissProt: Q53HV7 Human
    • Unigene: 632721 Human
    • Unigene: 731659 Human
    • Alternative names

      • FDG antibody
      • HMUDG antibody
      • MGC104370 antibody
      • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
      • Single strand selective monofunctional uracil DNA glycosylase antibody
      • Single-strand selective monofunctional uracil DNA glycosylase antibody
      • SMUG 1 antibody
      • Smug1 antibody
      • SMUG1 protein antibody
      • SMUG1_HUMAN antibody
      • UNG 3 antibody
      • UNG3 antibody
      see all

    Images

    • Flow Cytometry - Anti-SMUG1 antibody (ab15716)
      Flow Cytometry - Anti-SMUG1 antibody (ab15716)
      Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling SMUG1 with ab15716. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

       

    • Immunocytochemistry - Anti-SMUG1 antibody (ab15716)
      Immunocytochemistry - Anti-SMUG1 antibody (ab15716)

      Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).

      Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).

    • Immunocytochemistry - Anti-SMUG1 antibody (ab15716)
      Immunocytochemistry - Anti-SMUG1 antibody (ab15716)

      Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear and cytoplasmic staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).

      Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab15716? Please let us know so that we can cite the reference in this datasheet.

    ab15716 has been referenced in 2 publications.

    • Eldin P  et al. Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA. Nucleic Acids Res N/A:N/A (2013). PubMed: 24178031
    • Zaika E  et al. p73 protein regulates DNA damage repair. FASEB J : (2011). WB ; Human . PubMed: 21891782

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