Overview

  • Product name

  • Description

    Rabbit polyclonal to SMUG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SMUG1 (internal sequence). The exact sequence is proprietary.
    Database link: Q53HV7

  • Positive control

    • WB: SMUG1-transfected HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.

Target

  • Function

    Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FDG antibody
    • HMUDG antibody
    • MGC104370 antibody
    • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
    • Single strand selective monofunctional uracil DNA glycosylase antibody
    • Single-strand selective monofunctional uracil DNA glycosylase antibody
    • SMUG 1 antibody
    • Smug1 antibody
    • SMUG1 protein antibody
    • SMUG1_HUMAN antibody
    • UNG 3 antibody
    • UNG3 antibody
    see all

Images

  • All lanes : Anti-SMUG1 antibody (ab229868) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Human SMUG1-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS-PAGE gel.

References

ab229868 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229868.
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