Recombinant Anti-SMUG1 antibody [EPR15624] (ab192240)
![Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-415040-anti-smug1-antibody-epr15624-western-blot-hap1-wt-hap1-smug1-knockout-molt4-hek293.jpg "Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15624] to SMUG1
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SMUG1 antibody [EPR15624]
See all SMUG1 primary antibodies -
Description
Rabbit monoclonal [EPR15624] to SMUG1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SMUG1 aa 1-150. The exact sequence is proprietary.
Database link: Q53HV7 -
Positive control
- WB: HAP1, 293 and MOLT4 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab192240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23583 Human
- Omim: 607753 Human
- SwissProt: Q53HV7 Human
- Unigene: 632721 Human
- Unigene: 731659 Human
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Alternative names
- FDG antibody
- HMUDG antibody
- MGC104370 antibody
see all
Images
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Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SMUG1 knockout HAP1 cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDaLanes 1 - 4: Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. Wild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-230031-ab192240b.jpg)
Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-230030-anti-smug1-antibody-epr15624-western-blot.jpg)
Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa -
![Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-4-benefits-of-recombinant-antibodies.png)
Anti-SMUG1 antibody [EPR15624] (ab192240)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab192240 has not yet been referenced specifically in any publications.
