Recombinant Anti-SMUG1 antibody [EPR15624] (ab192240)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SMUG1 antibody [EPR15624]
See all SMUG1 primary antibodies -
Description
Rabbit monoclonal [EPR15624] to SMUG1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SMUG1 aa 1-150. The exact sequence is proprietary.
Database link: Q53HV7 -
Positive control
- 293 and MOLT4 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab192240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23583 Human
- Omim: 607753 Human
- SwissProt: Q53HV7 Human
- Unigene: 632721 Human
- Unigene: 731659 Human
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Alternative names
- FDG antibody
- HMUDG antibody
- MGC104370 antibody
see all
Images
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![Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-230031-ab192240b.jpg)
Immunoprecipitation - Anti-SMUG1 antibody [EPR15624] (ab192240)Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)](https://www.abcam.com/ps/products/192/ab192240/Images/ab192240-230030-anti-smug1-antibody-epr15624-western-blot.jpg)
Western blot - Anti-SMUG1 antibody [EPR15624] (ab192240)All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Datasheets and documents
References
ab192240 has not yet been referenced specifically in any publications.
