Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: Whole serum, 30% Glycerol
Our Abpromise guarantee covers the use of ab17919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/1000. Predicted molecular weight: 86 kDa. A nonspecific band is also observed at ~50kDa in HepG2 lysates.|
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 3 WW domains.
DomainThe second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
modificationsAuto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
Cellular localizationNucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
- Information by UniProt
- E3 ubiquitin-protein ligase SMURF2 antibody
- EC 6.3.2. antibody
- hSMURF2 antibody
ab17919 staining SMURF 2 in Xenopus Laevis cultured retinal ganglion cell growth cone tissue by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 21°C. The primary antibody was diluted 1/250 in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.
ab17919 has not yet been referenced specifically in any publications.