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    smurf-2-antibody-ab94483.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-SMURF 2 antibody (ab94483)

  • Datasheet
Reviews (2) Submit a question References (3)

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Western blot - Anti-SMURF 2 antibody (ab94483)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMURF 2 antibody (ab94483)

Key features and details

  • Rabbit polyclonal to SMURF 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SMURF 2 antibody
    See all SMURF 2 primary antibodies
  • Description

    Rabbit polyclonal to SMURF 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human SMURF 2 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab109647)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Caco2; MCF7; SHSY5Y; U20S; A549.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human SMURF 2 protein (ab95930)

Applications

Our Abpromise guarantee covers the use of ab94483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 3 WW domains.
  • Domain

    The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
    The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
  • Post-translational
    modifications

    Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
  • Target information above from: UniProt accession Q9HAU4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 427809 Chicken
    • Entrez Gene: 64750 Human
    • Entrez Gene: 66313 Mouse
    • Entrez Gene: 303614 Rat
    • Entrez Gene: 563633 Zebrafish
    • Omim: 605532 Human
    • SwissProt: Q9HAU4 Human
    • SwissProt: A2A5Z6 Mouse
    • SwissProt: A9JRZ0 Zebrafish
    • Unigene: 340955 Mouse
    • Unigene: 77630 Zebrafish
    see all
  • Alternative names

    • E3 ubiquitin-protein ligase SMURF2 antibody
    • EC 6.3.2. antibody
    • hSMURF2 antibody
    • MGC138150 antibody
    • Smad specific E3 ubiquitin ligase 2 antibody
    • SMAD specific E3 ubiquitin protein ligase 2 antibody
    • SMAD ubiquitination regulatory factor 2 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
    • SMUF2_HUMAN antibody
    • Smurf2 antibody
    • Ubiquitin protein ligase SMURF2 antibody
    see all

Images

  • Western blot - Anti-SMURF 2 antibody (ab94483)
    Western blot - Anti-SMURF 2 antibody (ab94483)
    Anti-SMURF 2 antibody (ab94483) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa
    Observed band size: 86 kDa
    Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-SMURF 2 antibody (ab94483)
    Immunocytochemistry/ Immunofluorescence - Anti-SMURF 2 antibody (ab94483)
    ICC/IF image of ab94483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab94483? Please let us know so that we can cite the reference in this datasheet.

    ab94483 has been referenced in 3 publications.

    • Li N  et al. Melatonin ameliorates renal fibroblast-myofibroblast transdifferentiation and renal fibrosis through miR-21-5p regulation. J Cell Mol Med 24:5615-5628 (2020). PubMed: 32243691
    • Li J  et al. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2. Cell Death Differ 26:2652-2666 (2019). PubMed: 31076633
    • Yan X  et al. Ginsenoside Rg3 Reduces Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor-ß1 by Inactivation of AKT in HMrSV5 Peritoneal Mesothelial Cells. Med Sci Monit 25:6972-6979 (2019). PubMed: 31527568

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-SMURF 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Melek Umay Tüz

    Verified customer

    Submitted May 05 2015

    Western blot abreview for Anti-SMURF 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cell line)
    Loading amount
    100000 cells
    Specification
    Breast cell line
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 21 2012

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